Former World Player of the Year Fabio Cannavaro was dropped by coach Ciro Ferrara on Saturday for the Serie A clash at home to AS Roma.
Juve come into the match in dire form with seven defeats in their last 10 matches and the Italy captain has paid the price for some dodgy recent performances in a defence that has looked anything but secure.
Nicola Legrottaglie replaces the 36-year-old who rejoined Juve in the summer following three years at Real Madrid.
Ferrara went for an attacking line-up with Alessandro Del Piero partnering Amauri in attack and playmaker Diego in the hole behind them.
Diego was the star when the teams met in Rome earlier in the season, scoring a brace in a 3-1 victory.
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