Lecce won 2-0 at Bari 2-0 on Sunday to relegate Sampdoria from Serie A, while Udinese won to retain fourth place.
A strike from Brazilian forward Jeda and Andrea Masiello’s own goal secured victory for Lecce against already-relegated Bari at the Stadio San Nicola.
Lecce remain 17th, but are now five points clear of third bottom Sampdoria, who lost 2-1 at home to Palermo.
Jonathan Biabiany had equalised for Sampdoria after Fabrizio Miccoli put Palmero ahead on the stroke of half-time.
But substitute Mauricio Pinilla struck with four minutes to go to see Palmero take all three points.
Coupled with Lecce’s victory, a defeat at home confirmed the end of Sampdoria’s eight-year stay in the top flight.
Like Bari, Brescia went into the penultimate round of matches already relegated to Serie B.
And the poor form that led to their demotion continued with a 1-0 defeat away to Cesena.
At the other end of the table, Udinese remain fourth and on course for a Champions League qualification berth after they beat Chievo 2-0 away from home.
Chilean Mauricio Isla and Ghana midfielder Kwadwo Asamoah were on target for Udinese.
With just one match of the season now remaining, they are two points clear of fifth-placed Lazio, who won 4-2 at home to Genoa.
Roma are out of top four contention after they lost 2-1 away to Catania.
Brazil international Robinho netted twice, while veterans Gennaro Gattuso and Clarence Seedorf were also on target, as champions AC Milan beat visitors Cagliari 4-1 at the San Siro.
Another disappointing season for Juventus took a turn for the worse with a 1-0 defeat at Parma, while Fiorentina were held 1-1 at home to Bologna.
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